Carbon Nanotube Conjugate with PEG as a Drug Delivery in to AMJ13 and HepG2 Cell
Marwa R. Jwameer†, Farah T. M. Noori‡, and Sabah A. Salman†
†Department of physics, college of science, university of Dayala, Iraq.
‡Department of physics, college of science, university of Baghdad, Iraq.
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In this study, a single well carbon Nanotunbe was used. The esterification reaction between the SWCNTs carboxylic acid group and the Polyethylene glycol (PEG) hydroxyl group was carried out using organic chemical materials (CNTs – COOH). PEG4000 was used to conjugate single well carbon nanotubes (SWCNTs) (medical polymer) and XRD, FTIR, UV- Vis, RS, and AFM were used to characterize the composite (PEG – CNTs). SWCNTS had a sharp peak at 291 nm in UV-vis, whereas (PEG – CNTs) had a peak at 289 nm. In Fourier transforms infrared spectroscopy (FTIR) spectra, a strong OH bond can be seen, as well as the C–H bond of (PEG-CNTs). CNTs have a sharp peak at 2 = 25.6299, which is related to 002 with d – spacing (3.4729 Å) while functionalization of CNTs – PEG 4000 conforms to a broad peak at 2θ= 23.4473 with d – spacing (3.8447Å). The values of crystalline size for CNTs and PEG-CNTs equals 3nm and 7.7nm, respectively. Atomic force microscopy (AFM) and CNTs images show a single well carbon nanotube with a grain size of 60 nm. In CNTs – PEG4000, the main grain size increases with functionalization to 83.60 nm, indicating aggregation of single well carbon nanotubes. In Raman Spectroscopy, the D and G bands in PEG-SWCNTs shift to (1288cm-1,1581cm-1) respectively. The inhibition rate of growth in cancer cells, including the breast cancer AMJ13 cell line and the liver cancer HepG2 cell line, was measured for 72 hours after they were exposed to a series of prepared (CNTs, PEG-CNTs) concentrations ((6.25- 100)mg/ml).The cytotoxicity screening showed that there was a highly toxic effect on the cancer cells. Gram-negative bacterial strain Pseudomonas aeruginosa and Gram-positive bacterial strain bacillus spp were exposed to a series of concentrations from prepared Platinum nanoparticles ((25-100) µl). The results exhibited significant inhibitory activity and that the rate of bacterial growth inhibition increased with increasing concentration.